桦褐孔菌多糖的提取及对肝癌细胞SMMC7721的抗增殖的研究

Extraction of Inonotus obliquus Polysaccharide and the Effect of Anti-proliferation on SMMC7721 Cells of Lung Cancer

探讨用超声提取法提取桦褐孔菌中桦褐孔菌多糖的工艺条件及桦褐孔菌多糖对肝癌SMMC7721细胞株的抗增殖作用。设计正交实验,对桦褐孔菌的超声提取工艺进行研究,并用MTT比色法研究桦褐孔菌多糖对肝癌SMMC7721细胞株的抗增殖作用。桦褐孔菌多糖超声提取的最佳工艺条件为95℃、15倍水、20kHz、超声60min,桦褐孔菌多糖在1.0￣16.0μg/mL范围内对肝癌SMMC7721细胞株的增殖均有抑制作用,且表现出浓度相关性。

OBJECTIVE To investigate the optimal purified protocol of extracting Inonotus obliquus polyseceharide using uhrasonication and the effect of the extractions from Inonotus obliquus polysaccharide on anti-proliferation for SMMC7721 cells of liver cancer. METHORD The method of orthogonal analysis was used to extract Inonotus obliquus polysaceharid with ultrasonieation, The methods of MTr was used to study the anti-proliferative effect of the polysaccharide on the hepatocarcinoma SMMC7721celIs. RESULT The best condition, used in the course of extraction of Inonotus obliquuspolysaecharide was： the temperature was 95℃, the volume of water was 15 times, the frequency of uhrasonication on the extraction was 20kHz, the optimal duration of extraction was 60rain. The Inonotus obliquus polysaccharide had the inhibiting action on SMMC7721 cells of liver cancer line in the concentration of 1.0-16.0ug/mL by means of MTr assay, and exhibited a strong dependence on concentration .