固相萃取-高效液相色谱法测定人血清中头孢吡肟浓度

Determination of cefepime in serum by solid phase extraction HPLC

目的：建立高效液相色谱法检测人血清中头孢吡肟浓度。方法：血清经固相萃取后，在Waters Symmetry shield RP18色谱柱上，以甲醇-20mmol·L^-1醋酸铵溶液（13：87）为流动相，流速1．0mL·min^-1，渡长检测254nm。结果：头孢吡肟的保留时间为6．82min，最低定量限为0．5mg·L^-1，线性范围0．5～100．0mg·L^-1，萃取回收率82．95％～86．25％，方法回收率98．05%～101．03％，日内RSD4．29％～9．83％，日间RSD5．21％～10．57％。结论：该法准确可靠，可用于头孢吡肟的临床药动学研究及，临床特殊人群的血药浓度测定。

OBJECTIVE To establish a new solid phase extraction HPLC method for determination of cefepime. METHODS Serum was extracted by a solid phase extraction（SPE） cartridge. The separation was performed on a Waters Symmetry shield RP18 （5μm, 250 mm×4. 6mm）column with mobile phase of methanol-20mmol·L^-1 ammonium acetate solution （13：87）. The flow rate was 1.0 mL·min^-1 and the detection was set at 254 nm. RESULTS The parameters of cefepime were as follows.ta was 6, 82 min; the determination limits of quantitation （LOQ） was 0. 5 mg·L^-1 in serum; the linear relation was in the range of 0, 5 - 100. 0mg·L^-1 the recovery of extraction was 82. 95%-86. 25%the recovery of methodology was 98. 05% - 101.03% the RSD for within-day and between-day were 4.29% - 9. 83% and 5.21% - 10. 57%, respectively. CONCLUSION The method could be used for pharraacokinetic studies and the determination of cefepime in serum in patients.