高效毛细管电泳法测定消炎抗菌片中没食子酸含量

Determination of the content of the gallic acid in the Xiaoyankangjun tablets by HPCE

目的：建立高效毛细管电泳法测定中成药消炎抗菌片中没食子酸的含量。方法：采用正交设计法优选了毛细管电泳的最佳参数，并根据选出的最佳条件的结果测定了消炎抗菌片中没食子酸的含量。定量采用内标法，选取苯甲酸钠为内标。所用毛细管规格为60cm（有效长度为45cm）×75μm，检测波长为215nm。结果：电压为21kV，电解缓冲液为10mmol·L^-1硼砂且pH为9．0，没食子酸在27．2～408．0mg·L^-1范围内线形关系起好（r=0．9999），加样回收率为99．03％，RSD为2．05%。结论：高效毛细管电泳法用于测定消炎抗菌片中没食子酸的含量是可行的，本方法简便、快速、准确、重复性好。

OBJECTIVE To establish an HPCE method for determining the gallic acid in Xiaoyankangjun tablets, METHODS The optimal condition of HPCE was determined by the orthogonal design method for the optimal conditon the contents determination of gallic acid in Xiaoyankangjun tablets sodium benzoate was used as internal standard. Fused-silica capillary total length 60 cm （effective length 45 cm,75 μm i. d. ）;detection wavelength： 215 nm. RESULTS applied voltage： 21 kV; electrolyte buffer： 10 mmol· L^-1 borate and pH 9. 0; The linear ranges of the gallic acid were 27. 2 - 408. 0 mg· L^-1 （r = 0. 999 9）. The recovery of the gallic acid was 99. 03%. The RSD was 2. （15%. CONCLUSION HPCE is a practicable method in the determination the concentration of gallic acid in the Xiaoyankangjun tablets.