北京市2001-2003年3141例儿科急性呼吸道感染中副流感病毒的研究

Parainfluenza virus infections in pediatric patients with acute respiratory infections in Beijing during 2001-2003

目的探讨应用传代狗肾细胞(MDCK细胞)进行副流感病毒(PIV)的分离,了解近年来北京地区儿科急性呼吸道感染中PIV的感染状况。方法收集2001年1月至2003年12月急性呼吸道感染患儿的临床标本3141例,应用微量细胞培养法比较MDCK细胞与传代猴肾细胞(Vero细胞)用于分离PIV的敏感性;所有标本接种于MDCK细胞,结合血凝试验及间接免疫荧光法进行PIV检测。结果Vero细胞PIV分离阳性的标本在MDCK细胞中也得到阳性结果;3141例标本中PIV阳性94例(3.0%)。1191例上呼吸道感染患儿标本中PIV135例(2.9%),PIV311例(0.9%);1634例下呼吸道感染患儿标本中PIV115例(0.9%),PIV324例(1.5%);207例支气管哮喘患儿标本中PIV阳性3例(1.4%);38例发热患儿中PIV阳性1例;71例其他标本中PIV阳性5例。结论利用MDCK细胞可进行PIV的分离;北京地区儿科急性呼吸道感染中PIV感染是原因之一。

Objective To understand the relationship of parainfluenza virus （PIV） and acute respiratory infections in infants and young children in Beijing, occurred in recent years. Methods 3141 throat swab/nasopharyngeal aspirate specimens were collected from infants and young children with acute respiratory tract infections in Beijing from Jan 2001 to Dec 2003. All of these 3141 specimens were inoculated into MDCK cells for influenza virus and PIV isolation, since PIV had been isolated in MDCK cells in this laboratory from preliminary studies. Out of 3141 specimens, 702 were inoculated into MDCK as well as Vero cells to compare the sensitivity on virus isolation of these cell lines by micro plate method. Growth of PIV in cell culture were identified by haemoagglutination test and indirect immunofluorescent assay. Results The PIV positive cases in Vero cells were also positive in MDCK cells, indicating that the sensitivity for PIV isolation in MDCK was equal to Vero cells. Out of these 3141 specimens, 94（3.0% ） were PIV positive, including 35 （3511191,2.9%） of PIV1, 11 （1111191,0.9%） of PIV3 in upper respiratory tract infections; 15 （1511634, 0.9%） of PIV1, 24 （2411634, 1.5%） of PIV3 in lower respiratory tract infections; 3 （31207,1.4 % ） of PIV in asthma; 1 （1138） of PIV in patients with fever; 5 （5/71） of PIV in others. Data indicated that among upper respiratory tract infections caused by PIV, PIV1 was more commonly seen than PIV3. Conclusion MDCK cells could be used for PIV isolation from clinical samples while PIV was one of the important pathogenic viruses causing acute respiratory tract infections in infants and young children in Beijing for the recente years.