摘要
利用穗茎注射法将反义PLDγ基因导入烟农15和农大1521两个小麦品种中,旨在获得抗病性增强的转基因植株。结果表明:T3代经PCR及PCR-Southern杂交鉴定,目的基因已经整合到小麦的基因组中,并能在小麦基因组内稳定遗传;烟农15和农大1521的6个转基因株系的抗白粉病的能力明显增强。
In order to obtain transgenic plants with enhanced powdery mildew resistance, antisense phospholipase Dγ（PLDγ） gene was introduced into two wheat cultivars Yannong 15 and Nongda 1521 by earstem injecting. By PCR and PCR-Southern analysis, anti-PLDγ gene had been integrated into wheat genomes of T3 generation with genetic stability. Powdery mildew resistance test showed that the ability of 6 transgenic lines were enhanced.
出处
《山东农业科学》
2006年第2期24-26,34,共4页
Shandong Agricultural Sciences
基金
山东省小麦良种产业化项目资助
关键词
小麦
反义PLDγ基因
白粉病
Wheat Antisense phospholipase Dγ gene
Powdery mildew
