基于电泳分离的生物芯片的设计与改进

Biochip Development and Improvement Based on Electrophoresis Separation

在一种低压驱动电泳分离的阵列电极模块基础上,设计制作了一款电泳分离用的基于MEMS技术的微流体沟道芯片,与电极模块及控制检测系统共同组成微全生物芯片系统。通过控制系统进行了一系列电泳实验,根据实验结果确定了沟道芯片的最佳尺寸参数。对阵列电极模块进行了改进,在上面增加了一对检测电极,通过比较几种检测方法选择对电泳分离实验进行阻抗检测,以实现检测系统的微型化。通过基于单片机的检测系统对电泳实验分离结果进行一系列的阻抗检测实验,实验结果证明检测电极的设计是可行的。

A Micro Fluidic Channel Chip was designed for electrophoresis separation using MEMS technology. After several electrophoresis experiments,the geometry parameters were finalized based on the analysis of the results. The low-voltage arrayed-electrode module also made great progress when a pair of detection electrodes were added to the module. By comparison among several detection technologies, the impedance detection method was chosen to realize the miniature system. The detection solution was further supported by the experiment results with the help of the impedance detection system.