仙人掌多糖的组成及其摩尔比测定

Component Analysis of Polysaccharide from Opuntia and Determination of its Molar Ratio

采用热水浸提法提取仙人掌多糖,多糖经去蛋白、柱层析分离得到两种组分OPA、OPB。OPA、OPB水解物经高效液相色谱及乙酰化气相色谱分析等,得到OPA由鼠李糖、阿拉伯糖、木糖、半乳糖、阿拉伯糖醛酸组成,其摩尔比为:1.13∶2.67∶1.00∶2.82∶1.02,OPB由鼠李糖、阿拉伯糖、葡萄糖、半乳糖、阿拉伯糖醛酸组成,其摩尔比为36.89∶3.56∶1.00∶10.55∶214.40。

The Polysaccharide can be extracted from Opuntia with hot water.Two main fractions OPA and OPB can be obtained by deproteining and purifying with column.The compositions of OPA and OPB can be separated by high efficiency liquid chromatography and gas chromatography,etc.The results show：OPA is composed of rhamnose,arabinose,xylose,galctose and arabinuronic acid with molar ratio of 1.13 ： 2.67 ： 1.00 ： 2.82 ： 1.02 and OPa is composed of rhamnose,arabinose,glucose,galctose and arabinuronic acid with molar ratio of 36.89 ： 3.56 ： 1.00 ： 10.55 ： 214.40.