过氧化物酶活性及其同工酶与葡萄对霜霉病抗性的关系

The relationship between activity of peroxidase and its isoenzymes and resistance of grapevine against downy mildew

采用盆栽接种的方法研究了不同抗病类型的葡萄品种感染霜霉病菌(Plasmopara viticola)后,过氧化物酶(POD)活性及同工酶酶谱的变化与葡萄抗霜霉病的关系.结果表明,霜霉病菌侵染后各品种的POD活性都有一个明显的消长过程,但抗病品种的酶活性升高的幅度大,持续的时间长.酶谱分析表明,不同抗性类型的葡萄感染霜霉病菌后,POD同工酶的数目、颜色与对照相比均有所增加.

The relationship between the activity of POD and its change of isoenzyme tables and the resistance to grapevine downy mildew with different disease-resistant types of grapevine varieties by Plasmopara viticola was studied through applying the method of pot culture inoculation. The result showed that there was a significantly change process in POD activity in infected varieties of grapevine by P. viticola,but the increased ranges of disease-resistant varieties of grapebine were larger and sustained time was longer. Analysis of enzyme tables showed： that both the quantity of POD isoenzyme and its colour increased compared with control group.