泌乳素对外周血淋巴细胞的免疫调节作用

Immunomodulatory Effects of Prolactin on Peripheral Blood Lymphocytes

目的探讨泌乳素(prolactin,PRL)对外周血淋巴细胞的免疫调节作用。方法采用ELISA法检测不同浓度PRL刺激外周血淋巴细胞分泌细胞因子的作用;应用流式细胞术分析不同浓度PRL对外周血淋巴细胞中T、NK细胞的影响。结果PRL刺激外周血淋巴细胞后,γ-IFN分泌明显增多,外周血中T、NK细胞比例变化不大,差异无显著性(P>0.05)。结论PRL作为免疫调节剂主要影响细胞因子的分泌。

Objective To explore the effects of PRL on peripheral blood lymphocytes. Methods The cytokines secreted by lymphocytes with different concentrations of PRL were investigated by ELISA kits; the proportions of T,NK subsets were detected by flow cytometric analysis. Results The secretion of γ-IFN was increased significantly, while the change of proportions of T,NK subsets were not significantly changed after the stimulation of PRL. Conclusions As the immunoregulators,PRL mostly modulates the cytokine secretion of lymphocytes.