薄层扫描法测定三种中药材中熊果酸的含量

Determination of the content of ursolic acid in three Traditional Chinese Medicines by the method of thin-layer chromatography

采用薄层扫描法对枇杷叶、白花蛇舌草、女贞子中熊果酸的含量进行了测定,其薄层扫描测定条件为:展开剂中环己烷-氯仿-乙酸乙酯(20∶5∶8);显色剂用10%H2SO4乙酸溶液。结果表明:在三种药材中,白花蛇舌草熊果酸含量最高,达0.896%;枇杷叶稍低,为0.843%;女贞子最低,为0.436%。该测定方法简便、快速,测定结果准确、可靠。

The content of ursolic acid in Eriobotrya japonica （Thtmb） L indl, Hedyotis diffusa Willd and Ligustrum lucidum Aitl were determinated by the method of thin-layer chromatography （ TIC）, the conditions of the determination by the method of TLC were as follow： the eluant was the mixture solution of cyclohexane, chloroform and acetic acid ethyl easter in the propotion of 20 ： 5 ： 8, and the color-developing agent was 10 % solution of H2SO4 in acetic acid. The results showed that the content of ursolic acid in Hedyotis diffusa Willd was 0. 896 %, which was the highest in the three medicines, and that in Eriobotrya japonica （Thunb） L indl was 0.843% ,which was a bit low,that in Ligustrum lucidum Aitl was 0.436 %, the lowest in the three. The determinafing method is easy to operate, fast, accurate and trustworthy.