秸秆双酶糖化条件试验研究

Study on the Straw Saccharification Conditions with Cellulase and Xylanase Simultaneously

利用双酶（纤维素酶、木聚糖酶）对秸杆直接糖化的条件进行了研究。结果表明：在纤维素酶1％、木聚糖酶0.01％、45℃、pH4．5、150rpm、液固比为15时，作用72h秸秆的糖化率最大。达到为10．815％：秸秆的糖化液中的还原性糖中主要有木糖和葡萄糖两种成份，比例分别为65％-68％和25％-33％。

The straw saccharification conditions with cellulose and xylanase simultaneously was studied. The result shows the maximum saccharification rate （ 10. 815% ） of straw was obtained under the conditions as follow：45 ℃, pH4.5,150 rpm, the ratio of liquid to solid is 15, the ratio of cellulase and xylanase to straw is 1% and 0.01% separately,and the saccharification time is 72 h. Glucose and xylose are the two main reducing sugar in the saccharification liquid of straw and their content are 65% - 68% and 25% - 33% separately.