三七总皂苷中各组分含量测定方法的改进

Improvement of determination method of the main components in Panax notoginseng saponions

目的用薄层扫描法（TLSC）和高效液相色谱法（HPLC）测定三七总皂苷中人参皂苷Rb1（Rb1）、人参皂苷Rg1（Rg1）和三七皂苷R1（R1）3种主要成分的含量，并对两种方法进行比较，为修订质量标准中含量测定方法及含量限度提供依据。方法TLSC法用正丁醇-乙酸乙酯-水（4：1：5）上层溶液为展开剂，27％硫酸无水乙醇溶液为显色剂，测定波长λs=535nm，λR=460nm；HPLC法用C18色谱柱，以乙腈-水线性梯度洗脱，0min（25：75）～15min（45：55）；流速1．5ml·min^-1；测定波长200nm。结果TLSC法测得三七总皂苷原料中Rb1、Rg1、R1的含量分别为31．07％、23．30％、9．35％；HPLC法测得三七总皂苷原料中Rb1、Rg1、R1含量分别为30．46％、22．65％、5．83％。结论HPLC法能将多种皂苷很好地分离并检测，简便快速，减少了误差。其准确度和测定结果的稳定性均优于TLSC法．

OBJECTIVE TLSC and HPLC were adopted to determine the contents of ginsenoside Rb1, ginsenoside Rg1 and sanchinoside R1 in Panax notoginseng saponions. And results of the two methods were compared, which could provide the basis of revising the determination method in quality standard. METHODS TLSC has been established with the upper layer of the mixture of butanol- ethyl acetate- H2O （4： 1：5） as developing solvent, and 27% sulphuric acid ethanol solution as coloring reagent, λS = 535 nm, λR = 460 nm. HPLC was adopted with C18 column, acetonitrile- H2O （25：75 at 0 min and 45：55 at 15 min, linear gradient elution） was used as mobile phase and detective wavelength was set at 200 nm. The flow rate was 1.5 ml·min^-1. RESULTS The content of ginsenoside Rb1, ginsenoside Rg1 and sanchinoside R1 in Panax notoginseng saponions determined by TLSC was 31.07%, 23.30% and 9.35% ; and that determined by HPLC was 30.46%, 22.65 % and 5.83 %, respectively. CONCLUSION HPLC could separate and determine various components in Panax notoginseng saponions and determine them. Its accuracy and stability are better than TLSC.