HPLC测定注射用甲硫氨酸维生素B_1中甲硫氨酸的含量

Content determination of methionine in Methionine and Vitamin B_1 for injection by HPLC

目的建立测定注射用甲硫氨酸维生素B1中甲硫氨酸含量的方法。方法采用HPLC法，固定相为氨基硅烷键合硅胶柱（250mm×4.6mm，5μm），流动相为乙腈-25mmol·L^-1磷酸盐缓冲液（60：40，pH3．5），测定波长210nm，流速0．8ml·min^-1。结果线性范围为0.128～0．300mg·ml^-1（r=0．9999），平均回收率为100．19％，RSD=0．70％（n=9）。结论所建方法简便、灵敏度高、重复性好。

OBJECTIVE To establish a method for determining methionine in Methionine and Vitamin B1 for injection.METHODS HPLC method was used and the seperation was performed on a column packed with amino chemically bonded silica（250 mm×4.6mm,5μm）. The mobile phase was acetonitrile- 25 mmol·L^-1 phosphate buffer solution （60：40,adjusting pH3.5）with flow rate of 0.8ml·min^-1 .The detective wavelength was set at 210 nm. RESULTS The linear range was good in 0. 128 ～ 0. 300mg·ml^-1 （ r = 0.9999）. The average recovery was 100.19% with RSD of 0.70% （ n = 9）. CONCLUSION The method is simple,sensitive and accurate with a high reproducibility.