COMT基因G1947 A突变与子痫前期的遗传易感性

The Study on the Association Between Catechol-o-methyltransferase Gene Polymorphism and Pregnancy-induced Hypertension

目的:探讨儿茶酚-O-甲基转移酶(COMT)基因G1947A突变与子痫前期遗传易感性的关系。方法:采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术对79例子痫前期患者及80例年龄与孕周皆相匹配的正常晚期妊娠对照者进行等位基因检测。结果:COMT基因G1947A位点G、A等位基因在子痫前期组频率分布分别为0·75、0·25,在对照组频率分布分别为0·71、0·29,两组间比较,差异无显著性(P>0·05)。COMT G/G、G/A、A/A基因型频率分布在两组间差异亦无显著性(P>0·05)。结论:COMT基因G1947A突变单独存在可能与子痫前期的遗传易感性无关,突变基因并没有增加子痫前期的发病风险。

Objective： The COMT gene contains a common functional polymorphism, Val158/108Met. The current study investigated the impact of this polymorphism on PIH risk. Methods： 102 patients with PIH and 94 normal controls matched for age and week of gestation were recruited. Genotyping was performed by using PCR - based restriction fragment length polymorphism （RFLP） method. Results： Significant differences were not observed between the PIH group and the control group in the frequencies of COMT alleles and genotypes. The genotype frequencies Of mild, moderate, severe PIH groups and control group are not significantly different. Conclusion： The COMT gene polymorphism is not associated with the generation of PIH and the severity of PIH. The COMT - L allele is not associated with the generation of PIH and the mutation genotype does not increase the risk of PIH.