摘要
目的检测ABCA1基因SNP,确定外显子16中V771M,T774P,K776N SNP发生率,并探讨它们与血脂代谢和冠心病易感性的关系。方法冠心病112例及健康者(对照组)108例,以PCR-SSCP法及测序法对第16号外显子进行多态性检测,并对其上的V771M,T774P,K776N位点进行限制性酶切。结果汉族人ABCA1基因V771M位点中仅有VV基因型和VM基因型,冠心病组VM基因型的发生率是5.4%,对照组发生率0.9%,冠心病组发生率高于对照组;冠心病组中VV及VM基因型之间比较血脂水平无明显差异(P>0.05)。结论汉族人AB-CA1基因外显子16中V771M位点M等位基因在不影响血脂水平的情况下增加冠心病的发生率。
Objective To study the characteristics of SNPs and the frequency distribution of V771M, T774P and K776N polymorphisms in the sixteenth exon of ABCA1 genc and to investigate its association with lipid metabolism and susceptibility to coronary artery disease. Methods SNPs in the sixteenth exon in ABCA1 gene were detected by PCR- SSCP and determined by sequencing. The seats of V771M,T774P and K776N in the sixteenth exon in ABCA1 gene were checked by restriction enzyme digestion. Results There were VV, VM genotypcs and no MM genotype in the scat of V771M. The frequency of VM ganotype was 5.4% in CAD group and 0.9% in control group. The difference of frequency distribution in VM genotypes is significant between CAD group and the control group ( P 〈 0.01). However, no significant difference was noted in total cholesterol, triglyceride, highdensity lipoprotein cholesterol and low - density lipoprotein cholesterol between the VM and VV genotypes in CAD group ( P 〉 0.05). Condusions The M allele of V771M SNP in the sixteenth exon of ABCA1 is associated with increased CAD without detectable changes in plasma lipids.
出处
《广东医学》
CAS
CSCD
北大核心
2006年第4期480-482,共3页
Guangdong Medical Journal
基金
国家自然科学基金资助项目(编号:30471929)
关键词
冠心病
三磷酸腺苷结合盒转运子A1
单核苷酸多态性
单链构象多态性
Coronary artery disease ATP binding cassette transporter A1 Single nueleofide polymorphisms Singlestrand conformation polymorphism