用于提取外周血DNA微流控样品预处理芯片的研制

Sample Pretreatment Microfluidic Chip for DNA Extraction from Rat Peripheral Blood

基于固相萃取原理和微电子机械系统(M icro-E lectro-M echan ical System,MEMS)技术研制了一种多孔氧化硅微流控样品预处理芯片,并利用具有大比表面积的多孔氧化硅作为提取DNA的固相载体,从而大大提高了DNA的提取产率.分析了影响DNA提取产率的因素,改进了芯片制备工艺和DNA提取实验方案,成功地提取了小鼠外周血DNA,提取产率为24 ng/(μL全血),达到商用试剂盒水平.同时以该DNA作为PCR扩增模板,扩增效果良好.

A sample pretreatment microfluidic chip was described based on the principle of solid phase extraction （SPE） and the MEMS technology. Oxidized porous silicon with a large surface area as the solid phase matrix for absorption DNA from biologic sample can greatly improve the DNA yield. The factors which can affect the DNA yield were analyzed and the preparation technology and the experiment procedure were improved. The DNA purification process from the rat peripheral blood can be achieved and the DNA yield is 24 ng/（μL whole blood）, which can reach the level of the commercial DNA purification kits. Furthermore, the DNA extracted from the whole blood can be amplified by PCR, which can achieve a high efficiency of the amplification.