人卵巢癌耐药细胞株的比较蛋白质组分析

Comparative Analysis of Proteomes for Chemoresistance in Human Ovarian Adenocarcinoma Cell Line,COC_1/DDP

The proteomic maps of human ovarian adenocarcinoma cell line in Human COC1 and its cell subline COC1/DDP were produced by 2-DE SDS-PAGE.The image analysis revealed obviously differential protein expression of the cells in response to cisplatin treatment.Approximate 1 300 protein spots were resolved in the two ranges of pI（3—10）.The spots which have 10-fold difference in light density were digested by in-gel（enzyme）,identified by matrix assisted laser desorption/ionization time-of-flight mass spectrometry（MALDI-TOF-MS） and finally detected by Profound and Mascot search engine in IDI databases.Seven proteins with obviously altered abundances were detected specifically,including four up-regulated protein spots and three down-regulated protein spots.The proteins differentially expressed in the parent cell line COC1 and its subline COC1/DDP were considered to be important for the therapy and pathogenesis.

The proteomic maps of human ovarian adenocarcinoma cell line in Human COC1 and its cell subline COC1/DDP were produced by 2-DE SDS-PAGE. The image analysis revealed obviously differential protein expression of the cells in response to cisplatin treatment. Approximate 1 300 protein spots were resolved in the two ranges of pl（3-10）. The spots which have 10-fold difference in light density were digested by in-gel enzyme, identified by matrix assisted laser desorption/ionization time-of-flight mass spectrometry （MALDITOF-MS） and finally detected by Profound and Mascot search engine in IDI databases. Seven proteins with obviously altered abundances were detected specifically, including four up-regulated protein spots and three down-regulated protein spots. The proteins differentially expressed in the parent cell line COC1 and its subline COC1/DDP were considered to be important for the therapy and pathogenesis.