十二烷基硫酸钠-水溶性非离子大分子间团簇化作用部位的~1H和^(13)C及2D NMR表征

Characterization of Clusters Interaction Sites at Cluster of Sodium Dodecyl Sulfate and Water Soluble Nonionic Macromolecules Investigated by ^(1)H NMR,^(13)C NMR and 2D NMR

运用1H NMR,13C NMR,2D NOESY-NMR及2D ROESY-NMR等多种波谱技术联合表征典型阴离子表面活性剂十二烷基硫酸钠(SDS)与典型水溶性非离子大分子聚乙烯吡咯烷酮(PVP)或聚乙二醇(PEG)间的团簇化作用部位.NMR综合分析结果表明,SDS分子亲水头基邻近的C1~C2片段与PVP分子中内酰胺氮为中心的主链α-C、五元环上羰基C1(记为P1)及C4(记为P4)片段给出较强的作用信号,推断SDS分子亲水头基与PVP分子中内酰胺氮及其两者的相邻区域可能为形成团簇时SDS束缚胶束与PVP间超分子作用的主要部位.而SDS束缚胶束与PEG形成团簇时,除极性部位及其相邻区域的相互作用外,部分PEG链节可能渗入到SDS束缚胶束的C3部位或更深的内部.

Clusters Interaction sites at the clusters of sodium dodecyl sulfate （SDS） and polyethylene glycol （PEG） and SDS-polyvinylpyrrolidone（PVP） were investigated by means of ^1H NMR, ^13C NMR, 2D NOESY-NMR and 2DROESY-NMR. The experimental results show that in SDS-PVP cluster, the interaction signal between C1 and C2 methylenes close to hydrophobic tail of SDS and the main chain α-C, carbonyl C1 segment （P1） and C4 methylene （P4） of the pentatomic ring around the central lactam N atom of PVP was very strong, which suggests that the probable interaction sites between PVP and the bound micelle of SDS are the hydrophilic head of SDS with the lactam N atom of PVP and the adjacent atoms of both molecules. Also in SDS-PEG cluster, besides the interaction of polar sections between SDS and PVP, some of PEG chains penetrate into the area nearby the C3 or deeper inside the bound mieelle of SDS.