甘蓝型油菜叶片与种子硫甙相关性研究

Study on the Relationship in Glucosinolate Between Leaves and Seeds of Brassica napus

【目的】研究甘蓝型油菜叶片与种子硫甙在不同生育时期的变化规律,分析叶片硫甙与种子硫甙相关性。【方法】采用高效液相色谱技术测定了甘蓝型油菜叶片与种子硫甙含量和组分。【结果】高硫、低硫甘蓝型油菜叶片硫甙含量变化规律显著不同,高硫油菜中油821叶片硫甙苗期含量最高,达33.15μmol·g-1,在随后的生育时期其硫甙含量持续下降;而低硫材料从越冬到现蕾逐渐升高,薹期降低,初花期又升高,盛花时降到最低。高硫品种中油821和其它低硫品系在薹期叶片硫甙含量接近,分别为7.63和6.55μmol·g-1,为甘蓝型油菜抗性改良和菜-油兼用型品种选育提供了参考依据。【结论】所有供试低硫材料叶片硫甙组分含量与种子硫甙组分含量间相关性均不显著,改变叶片硫甙含量不会显著影响种子中硫甙含量,脂肪族硫甙含量是高硫、低硫材料叶片硫甙总含量差异的主要原因。

[ Objective ] The variation principal in different growth periods of glucosinolates content was studied, and glucosinolate content correlations between Brassica napus leaves and seeds were discussed. [ Method ] Glucosinolates content and components were analyzed by high performance liquid chromatography （HPLC）. [ Result ] Leaf glucosinolate content in double-high material Zhongyou821 decreased from rosette to blooming stage, while in double-low material leaf glucosinolate increased from overwintering to budding period, decreased at stem elongation stage and rose again at early blooming stage. Leaf glucosinolate contents and profiles varied significantly in double-high and double-low rapeseeds. However, it was very close to each other in stem elongation period, and aliphatic glucosinolates were the dominant ones in both leaves. [Conclusion] There was no significant correlation in individual glucosinolate content between leaves and seeds in all double low rapeseed materials tested. Changes of glucosinolate contents in leaves will not affect glucosinolate content in seeds significantly.