三氯化铝对小鼠海马神经元细胞内游离钙浓度及其学习记忆的影响

Effect of aluminum trichloride on dissociated Ca2 ＋ in Hippocampus neuron cell as well as learning and memory

目的探讨接触不同浓度三氯化铝对小鼠海马神经元细胞内游离钙浓度的影响及其与学习记忆能力之间的关系。方法健康雄性ICR小鼠随机平均分为4组(1个对照组和3个染毒组),每组15只。对照组饮双蒸水,染毒组经饮水中加入三氯化铝染毒,剂量分别为10、50、300mg·kg-1·d-1,饲养100d,以钙敏感的荧光指示剂(Fura2)作为探针,观察三氯化铝对小鼠海马神经元细胞内游离钙的影响,利用Morris水迷宫实验测定小鼠学习记忆能力的变化。结果染铝50、300kg·kg-1·d-1剂量组海马神经元游离钙浓度([Ca2+]i)分别为[(412.25±53.20)、(467.37±32.85)次],与对照组[(293.91±32.21)次]相比明显下降,差异有统计学意义(P<0.01),且染毒剂量与[Ca2+]i呈正相关(rS=0.861,P<0.01);Morris水迷宫50、300mg·kg-1·d-1剂量组与对照组相比潜伏期延长,差异有统计学意义(分别为P<0.05和P<0.01)。结论铝对小鼠海马神经元细胞内游离钙浓度有影响,而其浓度的增加可能是导致小鼠学习记忆能力下降的原因之一。

Objective To investigate the effect of aluminum chloride on dissociated Ca^2 ＋ in hlppoeampus neuron cells in mice and the relationship to the learning and memory. Methods Male ICR mice in the three intoxicated groups were administered with the double distilled water containing AICl3 （ 10,50,300 mg·kg^-1·d^-1 ） while those in the control group were administered with the double distilled water for 100 days. The methods of behavior toxicology such as Morris swim maze were used for studying the effect of aluminum chloride on the changes of learning and memory in mice. With calcium sensitive fluorescence indicator Fura-2 as the fluorescent probe, the influence of the subchronic exposure to Al on the dissociated Ca^2＋ in hippocampus neuron cells was observed. Resatlts The dissociated Ca^2 ＋ in hlppocarnpus neuron cells in the middle dosage group and the high dosage group [ （412.25 ±53.20）, （467.37 ± 32.85）times] was lower than those in the control group [（293.91 ± 32.21） times] respectively （ P 〈 0.01）, and correlated positively with the dose and dissociated Ca^2＋ （ r = 0. 861, P 〈 0. 01）. Compared with the control group, the latent period was lengthened （ P 〈 0.05） in the middle dosage and the high dosage group. Conclusion The subchronic exposure to Alcl3 in mice affects the dissociated Ca^2＋ in hippccampus neuron cells. The increase of dissociated Ca^2＋ in hippocampus neuron cells may be correlated with the disfunction of cognition in the aluminium intoxicated mice.