分解菊粉微生物的筛选和初步鉴定

Screening and Primary Identification of Microbes Hydrolysing Inulin into Fructose

应用富集培养定向筛选技术，从菊芋根际土壤中分离到１８０余株分解菊粉的各类微生物．通过透明圈法初筛及摇瓶复筛，获得产菊粉酶能力最高的霉菌菌株ＡＪ１４０５，最高酶活可达１３·２μｍｏｌ·ｍｉｎ－１。

strains of microbes were isolated from soil samples by enrichment culture. Most of them were outmoded by means of examining whether there was a transparent area around the colony.Those with a larger transparent area were left, and then were further rescreened with shaking flask culture. At last 16 strains were obtained, the activity of producing inulinase was more than 3μmol/min. The maximum capacity of producing inulinase was about 13. 2μmol/min. Among the 16 strans,the stran AJ1405 was primarily identified. It was considered to be Aspergillus niger.