摘要
中药明党参用热水提取,乙醇沉淀后,经酶法和Sevag法脱淀粉、果胶和蛋白质,透析后上Sepharose-4B柱分离,得到两个多糖组份,CSP—1和CSP—2。两组分产率比是114。纸电泳、玻璃纤维高压电泳和醋酸纤维薄膜电泳都证明CSP—1和CSP—2都是均一组份。凝胶过滤法测得CSP—1和CSP—2的分子量分别是400KD和18KD。组成分析表明CSP—1含半乳糖和木糖,CSP—2含葡萄糖、鼠李糖和果糖。Discher法测试表明CSP—2含有糖醛酸。红外光谱和核磁共振波谱证明CSP—1和CSP—2都是α型糖苷键构型。高碘酸氧化和Smith降解分析表明CSP—1主要是(1→4)连接键。
Two polysaccharides ,CSP 1 and CSP 2,were obtained from Changium smyrnioides Wolff. They were proved to be homogenous by electrophoresis and gel filtration.Two ratio 1:4.M.W. of CSP 1 and CSP 2 were 500KD and 18KD respectively.CSP 1 and CSP 2 showed positive specific rotation: 18 D =+218(c=0.1,H 2O)for CSP 1, 18 D =+10,33(c=0.1,H 2O)for CSP 2.CSP 1 contained galatose and xylose,CSP 2 contained glucose,rahmnose and fructose.CSP 2 also contained uronuc acid indicated by Discher method.I.R.spectra and H NMR suggested that both CSP 1 and CSP 2 have α-glycoside linkage.From the results of pemodate oxidation and Smith detration,it was estimated that CSP 1 only had 14 linkage and that CSP 2 had 16 and 13 linkages.
出处
《安徽大学学报(自然科学版)》
CAS
1996年第3期95-100,共6页
Journal of Anhui University(Natural Science Edition)