发热患者SARS冠状病毒抗体分析

Detection of antibodies against SARS-associated coronavirus in patients with clinical fever

目的了解临床发热患者中是否存在严重急性呼吸综合征（SARS）冠状病毒（SARS-CoV）隐性感染或亚临床感染。方法对2003年5月～7月，SARS流行期间湖北地区临床发热患者373例血清进行SARS冠状病毒（SARS-CoV）抗体（IgG、IgM、N蛋白抗体）酶联免疫吸附法（ELISA）检测和分子生物学（RT—PCR）检测。结果373例发热患者中，检出冠状病毒抗体阳性者24例，阳性率6．43％（24／373），其中SARSIgG抗体、IgM抗体、N蛋白抗体阳性率分别为0．54％，1．34％和5．09％，但SARS-CoVRT—PCR检测均为阴性。结论临床发热患者可能存在SARS-CoV隐性或亚临床感染，患者体内产生针对SARS-CoV的保护性抗体，应具有免疫力和抵抗力。

Objective To determine whether there exist the inapparent infection or subclinical infection of severe acute respiratory syndrome associated coronavirus（SARS-CoV） among the patients with clinical fever. Methods 373 patients with clinical fever were selected during epidemic outbreak period of SARS from May to July 2003 in Hubei province. All the patients were received detection of specific SARS-CoV RNA and specific antibodies （ IgG, IgM and anti-N protein antibody） by RT-PCR and ELISA respectively. Results Specific antibodies against SARS-CoV （ IgG, IgM and anti-N protein antibody） were positive in 24 patients. The total positive rate was 6.43 % （24/373） and positive rate of IgG, IgM or anti-N protein antibody was 0.54 %, 1.34 % and 5.09 % respectively. However, the specific SARS-CoV RNA detected by RT-PCR assay was negative in the all patients. Conclusion The inapparent infection or subclinical infection of SARS-CoV may be existed in patients with fever. The patients may have immunity and resistance against SARS-CoV, because the protective antibodies directed against SARS-CoV are produced in the patients.