二月桂酸二丁基锡对大鼠肝脏毒性作用

Effect of dibutyltin dilaurate on hepatic toxicity in rats

目的用二月桂酸二丁基锡(DBTD)染毒大鼠,探讨DBTD对大鼠肝脏及血清中酶活性的影响。方法选健康成年大鼠28只,随机分为0,5,10,20 mg/kg 4个剂量组,每组7只动物,用DBTD灌胃染毒5周后处死动物,测定血清中碱性磷酸酶(ALP)、谷丙转氨酶(GPT)、γ-谷氨酰基转移酶(GGT)、肝组织中酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、乳酸脱氢酶(LDH)活性及肝脏中锡含量。结果各剂量组大鼠肝脏中锡含量,肝组织中ACP、AKP、LDH,血清中ALP、GGT、GPT活性明显高于对照组(P<0.01,P<0.05)。结论DBTD可在肝组织中蓄积,具有肝毒性,可影响肝中酶的活性及肝的正常功能。

Objective To study the effects of dibutyltin dilaurate（DBTD）on hepatic system and the activity of enzyme in serum of rats. Methods 28 health rats were chosen to do the experiment, and 7animals in each group. The rats were administrated orally with DBTD at the dose of 0, 5, 10 and 20 mg/kg respectively, and killed 5 weeks later. Alkaline phosphatases （AKP）, glutamic-pyruvic transaminase enzyme（GPT）and gamma glutamy ltransferase（GGT）in serum, and acid phosphatases （ACP）, alkaline phosphatases（AKP）, lactate dehydrogenase enzyme（LDH）, tin content in hepatic tissue were measured. Resuits The DBTD content of hepatic tissue was higher than that in control group（ P〈O. 05）, and the activities of ACP, AKP and LDH in hepatic tissue were higher than those in control group（ P 〈 0.05）, at the same time the activities of ALP, GGT and GPT were also obviously higher than those in control group（ P 〈 0.01 ）. Conclusion DBTD can accumulate in hepatic tissue and has hepatic toxicity, so it can affect the activity of enzymes in liver and the liver＇ s normal function.