纳米材料MCM-41的制备及其固定化酶的研究

Preparation of nanoporous material MCM-41 as well as immobilization of papain on it

在使用扩孔剂间三甲苯的条件下,对新型大孔纳米材料MCM-41进行了制备,并对该材料作为载体固定化木瓜蛋白酶进行了研究.X射线衍射和氮气吸附表征结果表明：材料内部孔的排列高度有序且孔径集中分布在6.3nm附近.用该材料作为载体固定化木瓜蛋白酶的结果表明：在给酶量、戊二醛质量分数、时间、温度和pH值分别为20mg/g,0.75%,2h,10～20℃和7.0的条件下,固定化酶的回收率为55%左右,效果最佳.

The present work dealt with the preparation of nanoporous material MCM-41 with mesitylene being a swelling agent as well as the immobilization of papain on the as-synthesized material with the aid of a coupling agent, glutaraldehyde. It was inferred from the diffraction data and the nitrogen sorption isotherms of the as-synthesized material that the material exhibited a highly ordered arrangement of uniform nanopores whose dimensions were determined to be around 6.3 nm. The optimum conditions for the immobilization were as follows： the concentration of native free enzyme, glutaraldehyde concentration, time, temperature and pH were 20 mg/g, 0.75%, 2h, 10-20℃ and pH 7.0, respectively. Under the optimum conditions, the activity yield （ % ） of the immobilized enzyme was around 55 %.