钙信使系统参与草酸对湖北海棠POD活性的诱导

Calcium signaling is probably involved in oxalic acid induced POD activity in Malus hupehensis(Pamp) Rehd. leaves

以EGTA、异博定(Ver)、氯丙嗪(CPZ)和L i+处理湖北海棠叶片,考察了钙信使系统在草酸诱导过氧化物酶(POD)中的作用。结果显示,4种抑制剂分别与草酸同时或先于草酸处理时,均明显抑制草酸对叶片POD总活性的诱导,表明Ca2+、Ca2+通道、钙调素(CaM)和磷酸肌醇均可能参与了草酸对POD的诱导。此外,草酸处理后一定时间再用抑制剂处理,也能够抑制草酸对POD的诱导作用。EGTA和Ver在草酸处理8 h后应用的效果低于4 h后应用;CPZ和L i+在草酸处理后4或8 h应用,效果相近。

The role of Calcium signaling in POD activity induced by oxalic acid in Malus hupehensis （Pamp） Rehd. leaves was studied by treating the leaves with Li^＋, EGTA, verapamil（Ver） and chlorpromazine （CPZ）. The results showed that these inhibitors could inhibit POD activity induced by oxalate when they were applied at the same time with oxalate or before oxalate treatment. The effect of the inhibitors and oxalate suggested that Ca^2＋ , Ca^2＋ -channel, Calmodulin （CaM） and inositol -1,4,5-tfisphosphate （ IP3 ） might be involved in the signal transduction by which oxalate induced POD activity. In addition, these inhibitors could inhibit POD activity induced by oxalate when they were applied after oxalate treatment. The effect of EGTA and Ver applied after 8 h was greater than that of them applied after 4 h of oxalate treatment on POD activity induced by oxalate. The effect of CPZ and Li^＋ applied after 4 h was similar with that of them applied after 8 h of oxalate treatment on POD activity induced by oxalate.