光催化H_2O_2氧化荧光法测定艾司唑仑

Study on the Fluorimetric Method of Estazolamvia H_2O_2 Oxidation with the Photocatalysis

研究了艾司唑仑在光催化下Ｈ２Ｏ２氧化产物的荧光性质，提出一种测定艾司唑仑的新方法．标准曲线的范围为０．６０～５．６０μｇ·Ｍｌ（－１），方法的相对标准偏差和检测限分别为２．１％和０．３１μｇ·ｍＬ（－１）．可直接用于片剂中艾司唑仑含量的测定．

Based on the photocatalyzed H2O2-oxidation of estazolam, which produces a fluorescent product (λes= 319 nm, λem= 424 nm ), a new method for the fluorimetric determination of estazolam was developed. The linear range of the calibration curve is 0. 60 ￣ 5. 60 μg. mL-1. The relative standard deviation and detection limit are 2. 1 % and 0. 3μg. mL-1, respectively. This method may be applied to the determination of estazolam in tablets.