摘要
应用碱变性法制备丝羽乌骨鸡(简称丝羽鸡)肝细胞mtDNA,测其分子量为18.5kb.用限制性内切酶进行酶切分析,结果表明,BamHⅠ、PstⅠ、HindⅢ、SacⅠ在丝羽鸡mtDNA分子上分别有1、1、1、2个酶切位点.根据单酶和双酶完全酶切片段的分子量,建立了丝羽鸡mtDNA的限制酶图谱.与由来亨鸡肝细胞mtDNA为材料所得的研究结果比较,发现丝羽鸡与来亨鸡在mtDNA种质方面存在着较大差异.
mtDNA from liver of Silkies was prepared by the alkaline lysis procedure. its molecular weight was measured to be 18. 5 kb, The results show that restriction endonucleaesBamH I, Pst I,Hind Ⅲ and Sac I have 1, 1, 1, 2 sites on the mtDNA molecule, respectively, uslug analysis of restriction enzyme cleavage. The restriction map of mtDNA from Silkies was constructed,according to the data of moleculat weight of generated fragments by complete digestion with single and double enzyme. mtDNAs of liver from Silkies and Leghorns have heredity variatin as compared with study results from Leghorns.
出处
《厦门大学学报(自然科学版)》
CAS
CSCD
北大核心
1996年第4期592-596,共5页
Journal of Xiamen University:Natural Science
基金
福建省自然科学基金