淀粉液化芽孢杆菌产β-葡聚糖酶培养基优化以及酶稳定剂的研究

Optimization of β-glucanase production medium with Bacillus amyloliquefaciens and β-glucanase stabilizer.

采用正交试验方法,进行了淀粉液化芽孢杆菌发酵产β-葡聚糖酶培养基的优化。结果表明,采用玉米粉30g/L,大麦粉40g/L,豆饼粉30g/L,Na2HPO4.12H2O 6g/L,(NH4)2SO4 4g/L,CaCl2 0.8g/L,MgSO4.7H2O1g/L组成的培养基发酵,β-葡聚糖酶活力达到128.55U/mL,比优化前提高了22.48%。在β-葡聚糖酶溶液中添加大分子亲水型多糖黄原胶、动物蛋白明胶、甘油、氯化钠可明显提高β-葡聚糖酶的热稳定性。将添加甘油120g/L、黄原胶5g/L复合稳定剂的葡聚糖酶溶液60℃处理2h,酶液的残余酶活比未经处理的酶活提高了55.3%。

Culture medium for Bacillus amyloliquefacie ns producing β-glucanase was optimized using orthogonal test method. The result showed that when culture medium was composed of corn flour 30 g/L, barley flour 40 g/L, soy flow 30g/L, Na2HPO4·12H20 6g/L, （NH4）2SO4 4g/L, CACl20.8 g/L, MgSO4·7H2O 1 g/L, the β-glucanase activity could reach 128.55 U/mL which was 22.48% increased. Adding giant molecule hydrophilic xanthangum, animal glutin, glycerol, NaCI into the β-glucanase solution, the thermal stability of β-glucanase could be increased. After addition of multiple stabilizer： glycerol 120 g/L, xanthangum 5g /L to the glucanase solution and 2 h 60℃ treatment, the enzyme activity was increased by 55.3%.