摘要
本文根据二级竞争酶联免疫定量测定的原理建立了用于乳牛早期妊娠诊断的二级竞争酶联免疫孕酮临界点判别分析法．本方法无需仪器比色和数学计算，反应结果清晰可辨、操作简便．根据对５２０头次配种后２１ｄ乳牛进行测试，其总正确率为８３．８％，其中阴性正确率为９０％，阳性正确率为７９％。
A double competition EIA was developed to measure progesterone for early pregnancy disgnosis of cows. By direct observing the result of reaction, the accurracy in assessment of pregnancy status by measurement of progesterone in skim milk of 520 cows collected at 21 days after artificial insemination,which was later confirmed by rectal palpation, was 79% for pregnancy and 90% for nonpregnancy.
关键词
二级竞争
酶联免疫
孕酮
乳牛
妊娠
诊断
Double competition
Enzyme immunoassay
Progesterone
Cow
Pregnancy diagnosis
