摘要
目的:设计一种旋转生物反应器并评价在其中进行细胞培养的效果。方法:制造一套旋转生物反应器并与静态培养进行比较。结果:扫描电镜结果表明旋转生物反应器动态培养的细胞生长速度、数量和形态明显优于24孔板静态培养。结论:自制旋转生物反应器可较好实现细胞三维立体培养,能作为细胞工程实验室一种较为理想的细胞培养装置,可在细胞和组织工程领域中广泛应用。
Objective： We have designed a rotating bioreactor and estimated the effect of it on cell culture. Method： We have made the rotating bioreactor and cultured cells in the bioreactor compared with static state. Result：The scanning electron microscope result indicated that growth- speed, quantity and modality in the bioreactor were better than that cultured in static 24 - pore plate. Conclusion：We developed the bioreactor can realize cell three- dimension culture, it is an ideal . equipment for cell culture, in cell engineering laboratory It can be used broadly to cell/tissues engineering.
出处
《激光杂志》
CAS
CSCD
北大核心
2006年第2期92-92,共1页
Laser Journal
基金
国家高技术研究发展计划(863计划)(2002AA326080)
四川大学创新资金
