食管癌组织中人乳头瘤病毒的检测

Detection of human papillomavirus in esophageal carcinoma

用人乳头瘤病毒（ＨＰＶ）保守基因的通用引物和１６、１８型特异性引物，通过聚合酶链反应（ＰＣＲ），检测２０例正常新鲜活检食管粘膜，４０例新鲜食管癌组织和５１例石蜡包埋的食管癌组织标本的ＨＰＶＤＮＡ，在２０例正常人食管粘膜组织中ＨＰＶＤＮＡ检出率９．１％，癌组织中为３６．２％，鳞癌和腺癌分别为３４．７％、４７．１％，鳞癌以１６型感染为主，腺癌以１８型为主。但肿瘤分化程度与ＨＰＶ感染无关。结果提示：ＨＰＶ感染与食管癌的发生有一定关系，不同型别的ＨＰＶ与不同组织的亲和性有差别。

Human papillomavirus (HPV) DNA was detected with general consensus primers and specific primers Type 16 and Type 18 through PCR from 20 normal and 40 esophageal carcinoma specimens of fresh biopsy and 51 specimens of paraffin-embeded esophageal carcinoma tissues.The detection rates of HPV DNA were 9.1% in the normal samples and 36.2% in carcinoma tissues.There were twenty five of 72 in squamous carcinoma (34.7%) and eight of 17 in adenous carcinoma (47.1%),and none in adenous-squamous carcinoma.HPV Type 16was predominate in squamous carcinoma and Type 18 in adenous carcinoma.There was no relation between the differentiation degree of carcinoma and the detecting rate of HPV DNA.