腹腔内热低渗液灌注化疗预防胃癌腹膜转移的实验研究和临床应用

Experimental Study and Clinical Application of Peritoneal Infusion of Hyperthermic and Hypotonic fluid with or without Anticancer Agent against Cancer Cell Implants

选用７０只ＬＡＣＡ。小鼠于其腹腔内植入Ｈ２２瘤细胞（每鼠瘤细胞数目２×１０７个）２４小时后，分别进行（４３Ｃ）单纯热低渗液、等渗液、ＤＤＰ及４３Ｃ低渗液ＤＤＰ腹腔内灌注治疗，观察其预防腹膜癌转移，延长生存期的作用。结果发现：以上方法均能破坏小鼠腹水瘤细胞，抑制腹水生长，阻止腹膜癌转移的发生（Ｐ＜０．０１），以热低渗液ＤＤＰ的作用最强（Ｐ＜０．０１），该组无１例发生腹膜癌转移。在实验研究的基础上，１９９１年９月～１９９２年９月对进展期胃癌根治性术毕采用预防性腹腔内热低渗液ＤＤＰ灌注治疗４６例，疗效满意。

In an attempt to prevent growth of peritoneal implant of cancer cells,infusion peritoneally with hyperthermic hypotonic chemotherapy was studied.LACA mice were given intraperitoneal of H22 H22 cancer cells(2×107 tumorcell,each mouse).24 hours later,intraperitoneal infusion of heated(43℃) hypotonic fluid or isotonic fluid or DDP alone or hypotonic fluid plus DDP were performed in different groups of mice.The results showed that lethal damage of the cancer cells in LACA mice peritoneal cavity was apparent and that the ascitic fluid was obviously halted and survival of the test LAtA mice was prolonged in all groups.However,in mice to which peritoneal hyperthermic hypotonic fluid infusion was given with DDP the effect was most prominent as compared with the other groups in terms of peritoneal tumor growth dissemination.Based on the experimental results intraperitoneal hyperthermic hypotonic fluid infusion containing DDP was performed on 46 patients with advanced gastric cancer after radical gastrectomy from September 1991 to September±1992.The results so far were gratifying.