粘附性淋巴因子活化的杀伤细胞抗自体白血病细胞作用的研究

Study on the Cytotoxicity of Adherent LAK Cells against Autologous Leukemic Blasts

建立了一套培养和处理粘附ＬＡＫ细胞（Ａ－ＬＡＫ）的系统，将１２例缓解期急性髓系白血病（ＡＭＬ）患者（ＲＰＳ）的Ａ－ＬＡＫ细胞与常规制备ＬＡＫ细胞（ＲＴ－ＬＡＫ）进行了对照研究，结果显示ＲＰＳ－Ａ－ＬＡＫ细胞于培养第１０天时其扩增指数（２０．１±１３．９）较ＲＴ－ＬＡＫ细胞的扩增指数（７．５±２．１）明显提高（Ｐ＜０．０５）。形态学研究显示Ａ－ＬＡＫ细胞主要由大颗粒淋巴细胞组成，免疫表型分析显示其主要由ＣＤ１６＋的ＮＫ细胞组成，ＲＴ－ＬＡＫ细胞主要由ＣＤ３＋的Ｔ细胞组成。其杀伤活性与ＣＤ１６＋ＮＫ细胞之间有很好的相关性（ｒ＝０．８２，Ｐ＜０．０５）。这提示ＣＤ１６＋ＮＫ细胞是Ａ－ＬＡＫ细胞的主要组成细胞，代表了杀伤功能最强的亚群。

A system for handling and culturing adherent LAK cells(A-LAK cell)was established,and the characteristic of A-LAK cells of 12 acute myelogenous leukemia patients(AML) in remission(RPs)wae studied in contrast to the LAK cells generated by conventional methods.The results indicated that the expansion index of RPs-A-LAK cells(20. 1±13. 9)was higher than that of conventional LAK cells(7.5±2. 1,P<0.05) and that its cytotoxicity was also higher(40.2±12. 1%VS 24.0±12.2%,P<0.05).MorPhology study indicated that A-LAK cells were mainly composed of LGLs.Immunophenotype study indicated that they were CD 16+NK cells and that their cytotoxicity correlated with the percentage of CD16+ NK cells(r=0.82 P<0.05).It is suggested that CD16+ Nk cells were the major component of A-LAK cells,and that they represented the most cytotoxic subset,