小鼠11个品系20个微卫星基因位点的遗传分析

Evaluation of STR Method for Mice Genetic Monitoring

目的利用PCR对近交系小鼠11个品系的20个微卫星基因座进行遗传检测,为实验室日常检测近交系小鼠的遗传背景提供一种分子生物学方法。方法用20个微卫星引物对11个品系近交系小鼠的基因组DNA进行PCR扩增。根据MMDBJ数据库信息,已知品系C3H、DBA、BALB/c和C57BL/6的多态性位点大小,由此可推知其他品系C57BL/6-Bg、C57BL/10、TA1、TA2、T739、M615和SCID的多态性位点大小。结果应出现的220条条带中有193条与预期的一致。结论利用这20个微卫星基因位点可以将11个品系的近交系小鼠区分开来,近交系小鼠具有丰富的遗传多样性,这些结果对于近交系小鼠的遗传检测具有重要指导意义。

Objective To analyze the polymorphisms of 20 mierosatellites in 11 strains of mice by PCR and to explore a new method for inbred strain mice genetic monitoring. Methods 20 short tandem repeat （STR） primers were used to amplify DNA in 11 inbred strains of mice （C3H, DBA, BALB/c, C57BL/6, C57BL/6-Bg, C57BL/10, TA1, TA2, T739, M615 and SCID）. The polymorphisms information of C57BL/6-Bg, C57BL/10, TA1, TA2, T739, M615 and SCID were acquired according to those of C3H, DBA, BALB/c and C57BL/6. Results 193 out of the 220 amplified bands were in anticipation. Conclusion The results showed that different stains of mice could be differentiated by use of polymorphisms of the 20 micmsatellites. It revealed that genetic diversity is abundant in inbred mice. These results have instructive significance for mice genetic monitoring.