不同剂量FSH和LH对小鼠超排效果的影响

Effect FSH and LH Administered in Different Doses on Mouse Superovulation

目的确定对昆明小鼠超排所需的FSH和LH最佳剂量,提高超排效果。方法48只小鼠随机分为四组,分别用3 IU6、IU9、IU、12IU的FSH分四次递减腹腔注射,48 h后分别注射LH 3 IU6、IU、9 IU1、2 IU,合笼;次日早检栓,48 h后冲胚。结果见栓率为79.17%(38/48);冲胚结果:3 IU FSH+3 IU LH6、IU FSH+6 IU LH9、IUFSH+9 IU LH、12 IU FSH+12 IU LH组平均每只小鼠获可用胚分别为5.67(68/12)枚、13.00(156/12)枚、29.17(350/12)枚、11.50(138/12)枚;且9 IU FSH+9 IU LH组与其余三组的超排效果差异均极显著(P<0.01)。

[ Objective To study the effect of FSH and LH on superovulation in KM mice. Method 48 mice were divided into 4 groups, which received FSH 3 IU, 6 IU, 9 IU and 12 IU i.p., respectively, quadrifidly and in a deereasing sequence. 48 hours later, LH in a dose of 3 IU, 6 IU, 9 IU and 12 IU was injected. The female mice were combined with male mice in one cages, and were checked for embolus the next morning. Embryos were washed out 48 hours later. Result The rate of embolus visualization was 79.17% （38/48）. The average of washing out embryos was 5.67 （68/12）, 13.00 （156/12）, 29.17 （350/12）, 11.50 （138/12） embryos per mouse according to the used hormones： 3 IU FSH ＋ 3 IU LH, 6 IU FSH ＋ 6 IU LH, 9 IU FSH ＋ 9 IU LH and 12 IU FSH ＋ 12 IU LH, respectively. Conclusion The protocol using 9 IU FSH ＋ 9 IU LH is the most effective to induce superovulation in KM mice among all schemes used in this study.