抗生素诱导的胃肠道菌群失调动物模型的建立

Animal Model of of Intestinal Microflora Dysbiosis Induced by Antibiotics

目的建立稳定的抗生素诱导的胃肠道菌群失调的动物模型。方法SPF级的BALB/c雌性小鼠随机分组,一组随机饮用0.5 mg/mL的头孢曲松溶液,一组灌胃125 mg/mL的头孢曲松溶液,连续4 d后检测盲肠菌群的变化,并且检测停用抗生素一周后菌群是否恢复正常。结果两种途径均能诱导菌群失调,且停用抗生素一周内难以恢复正常水平,但灌胃大剂量抗生素组小鼠的肠黏膜在一周内恢复较快,淋巴结的细菌移位率低于随机饮用抗生素组。结论饮用低浓度的抗生素是一种更优化的诱导菌群失调的方法。

Objective To establish an animal model of gastrointestinal microflora imbalance induced by an antibiotics, ceftriaxone. Methods Specific-pathogen-free mice received orally antibiotics in the drinking water ad lib （the concentration was 0.5 mg/mL）, or via intragastrical gavage in a dose of 125 mg/mL, 0.2 mL b.i.d, for 4 days. On the 5th day, the mice were sacrificed and intestinal flora analysis was performed. Results Both the two methods induced alteration of intestinal flora, but the intestinal mucosa of mice treated by intragastrical garage showed more rapid repair and the translocation of bacteria was lower than that in the mice of group administered with antibiotics in drinking water. Conclusion Administeration of ceftriaxone in drinking water ad llb is a perfect method to induced intestinal microflora dysbiosis in mice.