慢性病毒性心肌炎动物模型的建立

A Murine Model of Chronic Viral Myocarditis

目的建立小鼠慢性心肌炎实验动物模型,为心肌炎发病机理的研究以及治疗药物的筛选提供一个可靠的实验手段。方法采用BALB/c小鼠500只,用驯化的柯萨奇B3病毒进行感染,在实验的第10天、20天、40天8、0天和120天对实验小鼠进行各项实验数据检测。主要包括死亡率、病理形态、超微结构、心电图和血清特异性抗体等。结果①实验组小鼠死亡率为27.8%。②小鼠心脏重与体重比值随着实验时间的延长有明显的增加。③小鼠平均心肌炎发病率为49%,第120天仍有心肌病理改变。④实验小鼠心肌超微结构有明显改变。⑤实验小鼠异常心电图发生率约在50%以上。第120天实验小鼠心电图改变仍占57.7%。⑥小鼠血清特异性抗体IgG在各时间段变化不明显,而IgM随时间的延长阳性率有明显下降趋势。结论通过实验显示,一个稳定可靠的慢性小鼠心肌炎模型基本建立成功,本实验支持慢性心肌炎可导致扩张性心肌病的发生。

Objective To set up a murine model of chronic viral myocarditis and provide a reliable means for experimental study of pathogensis of viral myocarditis and drug screening for myocarditis treatment. Method 500 BALB/c mice were infected by trained CVB3. Experimental data were detected and collected at lOth d, 20th d, 40th d, 80th d and 120th d of the experiment. Results ① The mortality of mice in the experiment was 27.8 percent. ② The ratio of the mouse heart and body weight was significantly increased in the experimental group comparing to that of control group. ③ The myocarditis mobidity was 49% respectively in experiment group, There were still pathologic changes in the mice at 120th day, ④ Apparent ultrastructural changes were observed in myocytes. ⑤ The rate of abnormal ECG was above 50 per cent and 57.5% in the 120 d experiment mice. ⑥ The positive rate of serum specific antibody IgG was stable in the mice during the experiment, however, the positive rate of IgM decreased significantly along the experiment time from 10 to 120 days. Conclusion A chronic viral myocarditis model of mice has been established successfully. The data indicate that chronic viral myoearditis in the end stage can lead to occurrence of dilated cardiomyopathy.