屏障环境和独立通气笼盒(IVC)中大鼠生物学特性的比较

Comparison of Biological Features of SD Rats Bred in a Close System and Individually Ventilated Cages

目的大鼠在屏障环境和独立通气笼盒(Individually ventilated cages,IVC)中饲养条件下,某些生物学特性变化的比较。方法分别将10对SD大鼠在屏障环境和独立通气笼盒中饲养繁殖,观察生长发育和繁殖性能,测定50日龄仔鼠血常规、血生化指标。结果(1)大鼠生长发育及繁殖性能中胎间隔差异无显著性(P>0.05);屏障环境中雄性大鼠在30 d前较IVC中雄鼠增重明显,40 d后差异不显著;雌鼠在30 d以后差异显著。(2)血常规指标中血小板计数(PLT)、血小板比积(PCT)、血小板分布宽度(POW)、平均血小板体积(MPV)、大血小板(LPCR)差异有显著性(P<0.01);血生化中总胆红素(TBil)、直胆红素(DBil)、间胆红素(IBil)、碱性磷酸酶(ALP)、肌酐(Cre)、葡萄糖(Glu)、铜(Cu)、锌(Zn)、钠(Na)、氯(Cl)、总二氧化碳(CO2)升高(P<0.05或P<0.01),乳酸脱氢酶(LDH)、磷酸肌酸激酶(CK)、磷酸肌酸激酶同工酶(CK-MB)、低密度脂蛋白胆固醇(LCL-C)、载脂蛋白B(ApoB)、脂肪酶(LPS)、镁(Mg)下降(P<0.05或P<0.01)。结论在屏障环境和IVC条件下,大鼠的生长发育及某些血液学指标有一定的差异。

Objective To carry out a comparison of biological features of SD rats bred in a close system and individually ventilated cages. Methods The reproduction of 10 pairs of SD rats each bred in a close system or individually ventilated cages were evaluated, and the blood routine values and blood biochemical parameters of 50 days old rats bred in those two environments were examined and analyzed. Results （ 1 ） The interval between two births showed no significant difference （ P 〉 0.05）. The average weight of 0 ～ 30 days old male rats bred in IVC increased significantly faster, that of 40 ～ 50 days old male rats of the two groups increased with no significant difference （P 〉 0.05）. The average weight of 30 ～ 50 days old female rats showed a very significant difference （ P 〈 0.01 ） between the twogroups. （2） There were significant differences between the values of PLT, POT,PDW, MPV and LPCR in plasma between the two groups（ P 〈 0.01 ）. The levels of Tbil, ALP, Cre, GLU, Cu^2＋ , Zn^2＋ , Na^＋ , CI^- , CO2 increased significantly while LDH, CK, CK-MB, LCL-C, APOB, lipase, Mg2 ＋ decreased significantly （ P 〈 0. 05 or P 〈 0. 01 ） in the rats bred in close system. Conclusion Some differences exist as regards to the development and some blood indicators of rats bred in a close system or individually ventilated cages.