高效毛细管电泳－电荷耦合器件检测器联用技术研究（Ⅲ）──定量计算方法

Studies on the Coupling Technique Between High PerformanceCapillary Electrophoresis and Charge-Coupled Device Detector(Ⅲ)──Quantitative Calculation Methods

提出了加和峰高和峰体积法，并用于多波长电泳图的定量计算．将得到定性信息后的三维电泳图经峰加和等数据处理，转变成二维电泳图进行定量计算，提高了测定灵敏度、信噪比和计算结果的准确度与精密度．

This paper reports two calculation methods,namely,sum peak height and peakvolume,for quantitative calculation of multi- wavelength electropherogram.The three-di-mensional electropherogram,on which the quanlitative messages are obtained,is translatedinto a two-dimensional electropherogram by the use of such data processing procedures assummation of data in fluorescence spectra and so on,and the quantitative calculation is per-formed. The sensitivity of detection,signal to noise ratio, accuracy and precision of calculation result are therefore enhanced.