高效液相色谱法测定虫草杞芝颗粒中腺苷的含量

Determination of Adenosine in Chongcaoqizhi Granule by HPLC

目的建立一种反相高效液相色谱法测定虫草杞芝颗粒中腺苷含量的方法。方法以KromasilODS-1柱(250mm×4.6mm,5μm)为固定相;以磷酸盐缓冲液(pH6.5)-甲醇(85∶15)为流动相;流速为0.7mL/min;检测波长为260nm。结果腺苷对照品在1.016～12.192μg/mL范围内具有良好的线性关系,回归方程为Y=169339X-82318,r=0.9990,平均回收率为98.02%,RSD为1.72%;虫草杞芝颗粒中腺苷的平均含量为12.45μg/g。结论本方法结果准确可靠,重复性好,可用于虫草杞芝颗粒的质量控制。

Objective To develop a RP-HPLC method to determine adenosine in Chongcaoqizhi granule. Method The separation was performed in a Kromasil ODS-1 column （250 min×4.6 mm, 5μm） with a mobile phase of phosphate buffer solution （pH 6.5） - methanol （85 ： 15）. The flow was 0.7 mL/min and the detective wavelength was set at 260 rim. Results The standard adenosine showed a good linear correlation at range of 1.016- 12.192 μg/mL. The linear regression equation and correlation coefficient （r） were Y=169339X-82318, r=0.9990, respectively. The average recovery of the loading was 98.02% and the RSD was 1.72%. The average content of adenosine was 12.45μg/g. Conclnsion This method is accurate, reproducible and highly selective, and can be used for quality control of Chongcaoqizhi granule.