成年人前列腺组织细菌16S rRNA基因的检测

Detection of 16S ribosomal RNA gene of bacteria in prostate tissues of adults

目的 探讨慢性前列腺炎的细菌学病因，为临床治疗提供帮助。方法前列腺检测标本取自140例猝死的器官捐献者，年龄20～35岁。取前列腺周围带组织，分成两块，一块作常规病理检查，另一块用聚合酶链反应检测细菌16S rRNA基因。结果32．9％（46／140）的组织病理呈慢性前列腺炎，均为轻度灶性炎症改变，其中间质炎42例，间质伴腺体周围炎3例，腺体周围炎1例。16S rDNA阳性率为19．3％（27／140），其中前列腺炎标本阳性率为48．9％．（22／46），非前列腺炎标本阳性率为5．3％（5／94），前列腺炎标本阳性率高于非前列腺炎标本，差异有统计学意义（X^2=36．910，P〈0．001）。结论细菌感染可能是引起慢性前列腺炎的重要原因。

Objective To investigate the role of bacteria in chronic prostatitis. Methods Complete specimens of prostate were obtained from 140 organ donors, aged 20 - 35, at autopsy. A piece of tissue was collected from the peripheral zone of prostate from each specimen and was divided into 2 parts to undergo pathological examination and PCR so as to detect the 16S ribosomal RNA （ 16S rRNA ） gene of bacteria. Results Fecal mild inflammation was shown in 46 of the 104 specimens （32.9%）, including interstitial inflammation in 42 specimens, inflammation in both intersttia and body of gland in 3 specimens, and perigladulitis in 1 specimen. Twenty-seven of the 140 specimens （ 19.3% ） were positive in 16S rRNA gene. The positive rate of 16S rRNA gene of the specimens with prostatitis was 48.9% , significantly higher than that of the specimens without prostatitis （ 5.3%, P 〈 0. 001 ）. Conclusion Bacteria may play an important role in the pathogenesis of chronic prostatitis.