多参数流式细胞术在非霍奇金淋巴瘤诊断中的应用

Application of multiparameter flow cytometry in diagnosis of non-Hodgkin lymphoma

目的探讨流式细胞术在非霍奇金淋巴瘤(NHL)诊断中的应用意义。方法用多参数流式细胞术对40例淋巴组织增生性疾病的细针穿刺或手术切除淋巴结新鲜标本的活细胞表面抗原进行检测,同时结合细胞涂片的形态学观察,分析淋巴细胞的免疫表型特征,并与病理诊断及免疫组织化学结果进行比较。结果40例诊断为NHL的标本,经过流式细胞免疫分型联合细胞涂片观察细胞形态进行分析,37例(92·5%)符合NHL,其中病理组织学诊断为B-NHL的20例,经过流式细胞仪检测均为B-NHL,符合率为100%;病理组织学诊断为T-NHL的17例中,经过流式细胞仪检测符合T-NHL的12例(70·6%);2例(11·8%)修正为B-NHL;3例(17·6%)未能明确诊断。结论流式细胞免疫分型有助于提高NHL诊断的正确性和亚型分类的准确性。

Objective To evaluate the role and application of flow cytometry in the diagnosis of non-Hodgkin lymphoma （NHL）. Methods Fresh cell samples from 40 cases of lymphoproliferative disorders were obtained by fine needle aspiration or excisional biopsies. Multiparameter flow cytometry was used to study the surface antigens of lymphoid cells. The immunophenotyping results were also correlated with morphologic features seen in the cytology preparations. Results Of the 40 cases with histologic diagnosis of NHL, 37 cases （92. 5% ） had the lymphoma diagnosis confirmed by this method. The concordance rate for the 20 cases of B-cell NHL was 100%. As for the 17 cases with histologic diagnosis of T-cell NHL, 12 cases （66.7%） were correctly diagnosed as T-cell NHL using flow cytometry, while 2 cases （11.8%） were interpreted as B-cell NHL and the remaining 3 cases （17. 6%） were undiagnosed. Conclusion Immunophenotyping by flow cytometry can serve as an ancillary technique in diagnosis and subclassification of NHL.