摘要
目的探讨不同糖蛋白B(gB)基因型人巨细胞病毒(HCMV)感染与慢性牙周炎(CP)的关系。方法采用套式聚合酶链反应(nPCR)检测65例CP患者龈下菌斑标本和24名牙周健康者龈沟液标本中HCMV的gB基因,并进行限制性片段长度多态性分析(RFLP)对gB基因进一步分型。分析HCMV及其不同gB基因型感染与牙周炎严重程度的关系。结果以位点计,CP的HCMV感染率59·23%(154/260),明显高于牙周健康者的感染率32·29%(31/96)(P<0·01)。CP的HCMV感染者中gBⅠ型、gBⅡ型、gBⅠ和Ⅱ混合型分别占11·7%(18/154)、80·5%(124/154)和7·8%(12/154);牙周健康的HCMV感染者中gBⅠ型占45·2%(14/31),gBⅡ型占38·7%(12/31),gBⅠ和Ⅱ型混合感染占16·1%(5/31)。与牙周健康者相比,CP患者感染HCMV以gBⅡ型明显占多数(P<0·01)。不同gB基因型HCMV感染在不同临床附着丧失、探诊深度和牙龈指数的位点中的分布差异均无统计学意义(P>0·05)。结论HCMV感染与CP密切相关,但与牙周炎严重程度无直接关系。gBⅡ型可能是与CP相关的HCMV优势基因型。
Objective To investigate the correlation between infection of different human cytomegalovirus (HCMV) glycoprotein B (gB) genotypes and human chronic periodontitis. Methods A nested-polymerase chain reaction (nPCR) was employed to detect HCMV gB gene in the subgingival plaque samples from 65 chronic periodontitis patients and in the gingival crevicular fluid samples from 24 periodontally healthy control. The amplification fragments of gB gene were further genotyped by restriction fragment length polymorphism (RFLP). The correlation among infection with the different HCMV genotypes and the severity of periodontal lesion were evaluated. Results In tetras of teeth examined, the prevalence of HCMV (59. 23 % , 154/260) in the chronic periodontitis lesions was significantly higher than that of HCMV (32. 29 %, 31/96)in the periodontally healthy control (P 〈 0.01). Of the HCMV DNA positive samples from the chronic periodontitis lesions, 11.7 % (18/154) was genotyped as gBⅠ , 80. 5 % (124/154) as gB Ⅱ, and 7. 8 % (12/154) as gB Ⅰand gB Ⅱ co-infectioned, and of the HCMV DNA positive samples from the periodontal healthy control, 45.2 % (14/31) was genotyped as gB Ⅰ , 38.7 % (12/31) as gBⅡ, and 16. 1% (5/31) as gB Ⅰ and gB Ⅱco-infectioned. The gB Ⅱ genotype was more dominant among the chronic periodontitis lesions compared with that among the periodontally healthy control (P 〈0.01 ). In chronic periodontitis, no statistical significance could be found between infection of different HCMV gB genotypes and the different clinical parameters of CAL, PD and GI ( P 〉 0. 05 ). Conclusions Subgingival infection with HCMV is closely associated with chronic periodontitis. Infection of HCMV may not correlate directly with severity of periodontifis. However, gB Ⅱ may be the dominant genotype of HCMV, which is associated with the chronic periodontitis.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2006年第4期212-215,共4页
Chinese Journal of Stomatology
基金
浙江省科技计划基金资助项目(021107286)
关键词
牙周炎
巨细胞病毒感染
基因型
聚合酶链式反应
Periodontitis
Cytomegalovirus infections
Genotype
Polymerase chain reaction