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Slit-Robo信号对口腔癌Tb细胞凋亡作用的影响 被引量：3

Effect of Slit-Robo signal on apoptosis of oral cancer cell line Tb
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摘要 目的探讨Slit-Robo信号对人舌鳞状上皮癌细胞系Tb细胞凋亡的影响及其作用机制。方法以Robo1受体胞外区单克隆抗体R5作用于Tb细胞,采用克隆形成实验,流式细胞仪,DNA梯状电泳和Hochest-PI活细胞吸收分析法检测Tb细胞凋亡的发生和凋亡率,并通过Westernblot免疫印迹法检测凋亡相关蛋白fas和fasL的表达。结果在R5单克隆抗体作用下,Tb细胞增殖能力减弱,凋亡率增高(61·3%);fas和fasL蛋白表达水平上调,吸光度值分别为2022±243·989和2577±261·222。结论Slit-Robo信号可能抑制口腔癌Tb细胞的凋亡,这种抑制作用可能是通过fas-fasL蛋白的表达调控而实现的。 Objective To study the effect of Slit-Robo signal on apoptosis of human oral squamous cell carcinoma line Tb. Methods After the treatment in Tb cells with monoclonal antibodies （mAb） R5 of against Robol receptor extracellular domain, the apoptosis of Tb cell was examined by clone formation assay, flow cytometry, DNA ladder and Hochest-PI. The expression of fas and fasL proteins was observed by Western blotting analysis. Results After the treatment by R5 mAb, the proliferative rate decreased and the apoptotic rates increased, and the expression of fas and fasL proteins was up-regulated. Condusions SlitRobo signal could inhibit the apoptosis of tumor cells during genesis of tongue cancer by regulating the exDression of fas-fasL Droteins.
作者 马宇光 王丽京 韩冰 张洁
机构地区 兰州大学第一医院乳腺科 广东药学院基础医学院病理学教研室 兰州大学口腔医学院基础教研室
出处 《中华口腔医学杂志》 CAS CSCD 北大核心 2006年第4期232-235,共4页 Chinese Journal of Stomatology
基金 国家自然科学基金资助项目(30470858)
关键词 脱噬作用 口腔肿瘤 Slit—Robo信号 Tb细胞 Apoptosis Mouth neoplasms Slit-Robo signal Tb cell
分类号 R739.8 [医药卫生—肿瘤]
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参考文献10

  • 1Brose K,Bland KS,Wang KH,et al.Slit proteins bind Robo receptors and have an evolutionarily conserved role in repulsive axon guidance.Cell,1999,96:795-806.
  • 2Li HS,Chen JH,Wu W,et al.Vertebrate slit,a secreted ligand for the transmembrane protein roundabout,is a repellent for olfactory bulb axons.Cell,1999,96:807-818.
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二级参考文献8

  • 1Brose K, Bland KS, Wang KH,et al. Slit proteins bind Robo receptors and have an evolutionarily conserved role in repulsive axon guidance. Cell, 1999, 96 (6) :795
  • 2Li HS, Chen JH, Wu W,et al. Vertebrate slit, a secreted ligand for the transmembrane protein roundabout, is a repellent for olfactory bulb axons. Cell,1999, 96(6) :807
  • 3Wu W, Wong K, Chen JH, et al. Directional guidance of neuronal migration in the olfactory system by the concentration of the secreted protein Slit. Nature, 1999,400(6742) :331
  • 4Wu JY, Feng L, Park HT, et al. The neuronal repellent Slit inhibits leukocyte chemotaxis induced by chemotactic factors.Nature,2001,410(6831 ) :948
  • 5Wang B, Xiao Y, Ding BB, et al. Induction of tumor angiogenesis by Slit-Robo signaling and inhibition of cancer growth by blocking Robo activity. Cancer Cell,2003,4( 1 ) :19
  • 6Prasad A, Fernandis AZ, Rao Y, et al. Slit protein-mediated inhibition of CXCR4-induced chemotactic and chemoinvasive signaling pathways in breast cancer cells. J Biol Chem,2004,279(10) :9115
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  • 8吴军正,陈建元,李峰,李焰,张洪.舌癌脑转移细胞系Tb的建立及形态和生长特性[J].实用口腔医学杂志,1999,15(6):452-453. 被引量：12

共引文献11

同被引文献49

  • 1马宇光,王丽京,韩冰.Slit-Robo信号对口腔癌Tb细胞增殖作用的影响[J].实用口腔医学杂志,2005,21(4):441-444. 被引量：6
  • 2赵媛,王丽京,韩冰,马宇光,张洁.Slit蛋白在地鼠颊囊癌变过程中的意义及与血管形成的关系[J].实用口腔医学杂志,2006,22(3):361-364. 被引量：4
  • 3席银雪,宋鑫,陈惠新,彭挺生,陈旻湖.微血管密度在胃癌癌变多阶段组织中的表达及其意义[J].中华医学杂志,2006,86(48):3405-3408. 被引量：14
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  • 6Grone J, Doebler O, Loddenkemper C, et al. Rohol/Rdbo4: diffetential expression of angiogenic markers in colorectal cancer. Oncol Rep, 2006, 15:1437-1443.
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  • 8Rossi MR, Huntoon K, Cowell JK. Differential expression of the LGI and SLIT families of genes in human cancer cells. Gene, 2005, 356:85-90.
  • 9Dickinson RE, Dallol A, Bieche I, et al. Epigenetic inactivation of SLIT3 and SLIT1 genes in human cancers. Br J Cancer, 2004, 91:2071-2078.
  • 10Wang B, Xiao Y, Ding BB, et al. Induction of tumor angiogenesis by Slit-Robo signaling and inhibition of cancer growth by blocking Robo activity. Cancer Cell, 2003,4:19-29.

引证文献3

二级引证文献3

中华口腔医学杂志
2006年 第4期

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