肠日本血吸虫病与肠癌组织c-erbB-2及DPC4表达水平的研究

Investigation of expression of c-erbB-2 and DPC4 in tissues of colonic schistosomiasis and colorectal carcinoma

目的探讨慢性日本血吸虫病与大肠癌之间的关系。方法用免疫组化两步法及SP法分别检测1 5例正常结肠黏膜,1 5例单纯血吸虫病结肠黏膜,2 0例血吸虫病并大肠癌,2 0例无血吸虫病大肠癌的c-erbB-2及DPC 4的表达。结果c-erbB-2在4组中均有一定程度的表达,但在血吸虫病肠黏膜中的表达最高(P<0.0 5)。DPC 4亦在4组中有一定程度的表达,但在正常肠黏膜的表达水平最高(P<0.0 5)。结论慢性日本血吸虫病与大肠癌之间存在一定的关系;慢性日本血吸虫病引起黏膜上皮恶变时,c-erbB-2,DPC 4表达水平的改变可能是一早期分子事件。

Objective To determine the relationship between chronic colonic schistosomiasis and colorectal carcinoma. Methods Expression levels of c-erbB-2 and DPC4 were detected by two-step and SP immunohistochemical method respectively in normal colonic mucosa of 15 cases, in colonic mucosa of 15 cases with simple schistosomiasis, 20 cases of colorectal carcinoma with schistosomiasis and 20 cases of colorectal carcinoma without schistosomiasis. Results c-erbB-2 was expressed in each group with different levels, but the level in colonic mucosa with schistosomiasis was the highest compared to other groups, which had statistical significance （ P 〈 0.05 ）. DPC4 was also expressed in each group with different levels, but the level in normal colonic mucosa was the highest compared to other groups, which had statistical significance （ P 〈 0.05 ）. Conclusions There is a high correlation between chronic schistosomiasis japonica in colon and colorectal carcinoma. Alteration of expression levels of c-erbB-2 and DPC4 may be an early stage molecular event during the course of malignant change of mucosal epithelium caused by chronic colonic schistosomiasis.