利用原位杂交技术研究SIPAR在小鼠不同发育阶段的表达

SIPAR EXPRESSION PATTERN DURING THE DEVELOPMENT OF MOUSE EMBRYO DETECTED BY WHOLE MOUNT IN SITU HYBRIDIZATION

目的检测对STAT3信号通路有调节作用的新基因SIPAR（STAT3 interacting protein as a represor）在小鼠胚胎发育过程中不同阶段的表达.方法采用地高辛标记探针的整体胚胎原位杂交方法.结果 SIPAR在dpc9.5的小鼠胚胎眼泡和听泡中有明显的表达,在dpc9.5～12.5的小鼠胚胎脊柱神经和脑部中枢神经都有表达,在dpc12.5小鼠胚胎脑、眼泡和脊柱中表达增强.结论 SIPAR主要集中表达在小鼠胚胎的神经系统,SIPAR可能与神经系统发育相关.

Objective A new gene SIPAR（STAT3 interacting protein as a repressor） is involved in the regulation of STAT3 signal pathway. To study SIPAR expression pattern in mouse embryonic development. Methods Using the whole mount in situ hybridization with a digoxigenin labeled probe. Results The results indicated that SIPAR was expressed in otic vesicles and eye vesicles obviously in mouse dpc9.5 embryo. SIPAR continued to expressed in the mouse embryonic brain and vertebral column part from dpc9.5 to dpc 12.5 embryo. Furthermore,the expression was enhanced in brain,eye vesicles and vertebral column in dpcl2.5 embryo. Conclusion SIPAR is mainly expressed in nervous system including brain, otic vesicles, eye vesicles and vertebral column in mouse dpc9.5-dpcl2.5 embryo. Due to the important function of STAT3 signal pathway in nervous system development,whether SIPAR has effect on STAT3 activity in the nerve system will be further investigated.