Using p53-immunostained large specimens to determine the distal intramural spread margin of rectal cancer

瞄准：决定直肠的癌症的远侧的内部传播(阴间的神) 边缘。方法：直肠的癌症的 61 个 p53 积极的标本被使用。在染色的常规苏木精和曙红(H&E ) 以后，在大标本的直肠的癌症的 DIS 边缘被免疫组织化学检验。病人被划分成 A， B， C，和 D 组。在长期的后续以后，四个组的幸存曲线用寿命表被估计。结果：61 个盒子(83.6%) 中的 51 个有阴间的神。阴间的神的程度变化了 0.11-3.5 厘米；同时，阴间的神的平均数由染色的 H&E 测量了是 0.13 厘米。有效差量在工具之间被发现(t=5.622， P【0.0001 ) 。仅仅， 51 个病人中的 1 个有比 3 厘米大的阴间的神。阴间的神是在很直肠的癌症病人的不到 1.0 厘米。长期的结果显示其阴间的神比 1.0 厘米大的病人的幸存率比其阴间的神是不到 0.5 厘米的病人的低。结论：有比 1.0 厘米大的阴间的神的直肠的癌症病人有差的预后。

AIM： To determine the distal intramural spread （DIS） margin of rectal cancer. METHODS： Sixty-one p53-positive specimens of rectal cancer were used. After conventional hematoxylin and eosin （H＆E） staining, the DIS margin of rectal cancer in large specimens was examined by immunohistochemistry. The patients were divided into A, B, C, and D groups. After a long-term follow-up, the survival curves of the four groups were estimated using the life table. RESULTS： Fifty-one of the sixty-one cases （83.6%） had DIS. The extent of DIS ranged 0.11-3.5 cm; meanwhile the.mean of DIS measured by H＆E staining was 0.13 cm. The significant difference was found between the means （t=5.622, P〈0.0001）. Only 1 of 51 patients had DIS greater than 3 cm. The DIS was less than 1.0 cm in most rectal cancer patients. The long-term results indicated that the survival rate of the patients whose DIS was greater than 1.0 cm was lower than that of the patients whose DIS was less than 0.5 cm. CONCLUSION： Rectal cancer patients with DIS greater than 1.0 cm have poor prognosis.