基因放射性核素反义治疗肾癌的体内外实验

The Effect of Radionuclide Antisense Therapy Targeting Ki67 Gene on Proliferation and Apoptosis of the Human Renal Carcinoma Cells in vitro and in vivo

目的:探讨Ki-67基因放射性核素反义治疗对人肾癌细胞生长及凋亡的影响。方法:125I标记的反义核酸(125I-ASODNs)处理人肾癌786-0细胞及荷瘤小鼠,采用免疫组化、Westernblot检测Ki-67表达;TUNEL法检测细胞凋亡。结果:肾癌细胞Ki-67抗原表达降低、Ki-67蛋白降低、凋亡细胞阳性率升高、小鼠肿瘤体积减小。结论:Ki-67基因放射性核素反义治疗能抑制肾癌细胞Ki-67基因表达及细胞增殖、促进凋亡。

Objective： To evaluate the effects of ^125I-labeled antisense oligonucleotide （^125I-A- SODNs） targeting Ki-67 gene on the proliferation and apoptosis of human renal carcinoma cell line in vitro and in vivo. Methods： Antisense oligonucleotides （ASODNs） was coupled with ^125I. Human renal carcinoma cell line 786-0 cells and implanted tumor mice were treated with ^125I-ASODNs. The Ki-67 expression was detected using immunohistochemical technique and Western blot method, respectively. Apoptosis of 786-0 cells was detected by TUNEL assay. Results： The Ki-67 positive expression rate and Ki-67 protein rate were low, but the rate of apoptosis was high. The tumor volume was significantly small. Conclusion： Effects of ASODNs targeting Ki67 gene on the proliferation and apoptosis of human renal carcinoma cells are augmented when ASODNs coupled with ^125I.