肿瘤坏死因子-α和干扰素-γ多态性与婴儿巨细胞病毒肝炎的关系

Relationship between Tumor Necrosis Factor-α and Interferon-γ Polymorphism with Infant Cytomegalovirus Hepatitis

目的探讨肿瘤坏死因子-α(TNF-α)基因+238位点和+308位点G/A及干扰素-γ(TFN-γ)+874位点A/T单核苷酸多态性与婴儿巨细胞病毒(CMV)肝炎的关系。方法对CMV肝炎患儿87例和同期入院非CMV肝炎患儿89例,应用ABIPrism7700高通量荧光PCR系统进行测定TNF-α基因+238和+308及TFN-γ+874位点单核苷酸多态性。结果婴儿CMV肝炎患儿TNF-α基因+238和+308位点与对照组比较无显著性差异。IFN-γ+874位点在婴儿CMV肝炎患儿中AA型64例,AT型20例,TT型3例;对照组AA型45例,AT型26例,TT型18例,两组基因型和A/T等位基因频率分布存在显著差异(P=0.001,P<0.001)。结论IFN-γ单核苷酸多态性与婴儿CMV肝炎的易感性有一定的相关性;TNF-α+238和+308单核苷酸多态性与CMV肝炎易感性可能无关。

Objective To explore the relationship between tuinor necrosis factor（TNF）-α promoter G238A,G308A and interferon （IFN）-γ＋874A/T polymorphism and susceptibility to cytomegalovirns（CMV） hepatitis, Methods A TaqMan fluorescence polymerase chain reaction （PCR） in the IFN-γ＋874A/T and TNF-α gene promoter region single nuclcotide polymorphism was tested in the subjects, including 87 infants with CMV hepatitis and 89 children without CMV hepatitis. Results No evident difference of TNF-α＋238G/A and ＋ 308G/A allele frequency was found between infants with CMV hepatitis and controls. The cases of IFN-γ＋ 874 AA, AT and TT genotype were 64,20,3 cases in the infant CMV hepatitis group, and 45,26 and 18 cases in the control group, respectively, There was significant difference in IFN-γ＋ 874 allele genotype and frequency between infants with CMV hepatitis and the control group（P = 0.001 ,P〈0.001）.Conclusions There is significant relationship between IFN-γ＋874 polymorphism and susceptibility to CMV hepatitis, TNF-α promoter G238A and G308A polymorphism are not associated with CMV hepatitis.